Written by Yvette Tran (PEACE Officer Intern)
What is the nucleotide sequence of the DNA strand that is complementary to 5’-ACGCTTACGTA-3’?
a. 5’-TACGTAAGCGT-3’
b. 5’-AACGTAACGCT-3’
c. 5’-TACCTAAAGCT-3’
d. 5’-TGCGAATGCAT-3’
e. 5’-TFCGTTAGCAT-3’
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Explanation: The answer is A. It is important to remember that DNA strands within a double helix run antiparallel to each other and follow Watson & Crick’s base pairing rules. In doing so, A pairs with T via 2 hydrogen bonds and C pairs with G via 3 hydrogen bonds. (Helpful mnemonic to remember: Chips always go with guacamole)