Written by Yvette Tran (PEACE Officer Intern)
When visualizing a TLC plate, how can you tell if a spotted solution is pure or impure? On that same note, how can you tell if a spotted solution is more polar or less polar than another spotted solution?
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Click “Read More” to see the answer.
Pure compounds will be present on the TLC plate as well-defined distinct spots whereas impure solutions will result in several spots present on the TLC plate. More polar solutions will have a lower Rf value than less polar solutions.